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| Registros recuperados: 116 | |
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| Codevilla,Cristiane Franco; Tischer,Bruna; Gindri,Amanda Leitão; Nogueira-Librelotto,Daniele Rubert; Barin,Juliano Smanioto; Silva,Cristiane de Bona da; Rolim,Clarice Madalena Bueno; Zepka,Leila Queiroz; Menezes,Cristiano Ragagnin de. |
| ABSTRACT: The high intensity ultrasound-assisted extraction (HIU) is one of the most simple, quick and efficient techniques for the extraction of phenolic and other antioxidant compounds from plants. This is the first application of HIU for the extraction of these compounds from goldenberry fruit. The HIU and conventional extraction techniques showed similar results regarding to phenolic compounds and antioxidant capacity. However, the time required for HIU extraction (5min) was 24 times lower than conventional extraction (120min). Phenolic compounds reported were chlorogenic acid, caffeic acid and rutin. In vitro cytotoxicity assays were used for evaluation of extracts and the results showed that in a wide range of concentration, the extract maintains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goldenberry; Sonication; Antioxidant capacity; Cytotoxicity; High performance liquid chromatography. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200752 |
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| Mizuno,Cassia Suemi; Ampomaah,Winnifred; Mendonça,Fernanda Ribeiro; Andrade,Gabriela Carvalho; Silva,Ariel Maria Nazaré da; Goulart,Mirian Oliveira; Santos,Raquel Alves dos. |
| Abstract The cytotoxicity and genotoxicity of the stilbenes (E)-methyl-4-(3-5-dimethoxystyryl)benzoate (ester), (E)-4-(3-5-dimethoxystyryl)aniline (amino), (Z)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (cis-TMS) and (E)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (trans-TMS) were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses. The parameters were evaluated using XTT assay, clonogenic survival assay and the cytokinesis-block micronucleus assay in CHO-K1 and HepG2 cell lines. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stilbene; Cytotoxicity; Genotoxicity; Micronucleus assay. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400656 |
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| Bustillo,S; Lucero,H; Leiva,LC; Acosta,O; Kier Joffé,EB; Gorodner,JO. |
| Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 µg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50: 2 µg/mL)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops alternatus; Bothrops diporus; Cytotoxicity; C2C12; Apoptosis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100004 |
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| Geiger, Marie; Guitton, Yann; Vansteelandt, M.; Kerzaon, I.; Blanchet, Estelle; Du Pont, T. Robiou; Frisvad, J. C.; Hess, Philipp; Pouchus, Y. F.; Grovel, Olivier. |
| In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with... |
| Tipo: Text |
Palavras-chave: Cytotoxicity; Dereplication; Marine fungi; Mass spectrometry; Penicillium; Shellfish. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00160/27166/25523.pdf |
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| Linan,Lamia Zuniga; Lima,Nadson Murilo Nascimento; Benatti,Carol; Xavier,Mariana; Rodrigues,Ana A.; Manenti,Flavio; Jardini,Andre; Maciel Filho,Rubens; Gilioli,Rovilson. |
| ABSTRACT A cytotoxicity study is performed on a poly(methyl methacrylate) polymer (PMMA) to be used for the fabrication of bone tissue by Rapid Prototyping (RP). The solution polymerization is conducted in a pilot plant reactor using more appropriated reagents in consideration of the medical application. Moreover, the polymer is efficiently handled to avoid the side effect of the monomer, reducing the concentration of this specie to 287,731 µg MMA/kg PMMA. The cytotoxicity of the polymer is determined through growth monitoring, adherence and morphology of L-929 cells. Additionally, MTT and LIVE/DEAD tests are performed. The results showed continuous and progressive growth of the cells on the surface of the specimens. Moreover, the material did not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PMMA; Synthesis; Cytotoxicity; Viability; Mesenchymal cells.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100601 |
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| Dzoyem,Jean P.; Tsamo,Armelle T.; Melong,Raduis; Mkounga,Pierre; Nkengfack,Augustin E.; McGaw,Lyndy J.; Eloff,Jacobus N.. |
| BACKGROUND: Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric oxide (NO) and acetylcholinesterase (AChE) inhibitory activity and the cytotoxicity of three limonoids: trichilia lactone D5 (1), rohituka 3 (2) and dregeanin DM4 (3), isolated from Trichilia welwitschii C.DC RESULTS: Results indicated that the three limonoids had low cytotoxicity towards Vero cells with LC50 values ranging from 89.17 to 75.82 (μg/L. Compounds (2) and (3) had lower cytotoxicity compared to puromycin and doxorubicin... |
| Tipo: Journal article |
Palavras-chave: Trichilia welwitschii; Cytotoxicity; Acetylcholinesterase; Nitric oxide; Limonoids. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100057 |
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| Jamunaa,A; Vejayan,J; Halijah,I; Sharifah,SH; Ambu,S. |
| Cytotoxicity of venoms from eleven medically important snakes found in Southeast Asia (Naja kaouthia, Naja siamensis, Naja sumatrana, Ophiophagus hannah, Bungarus candidus, Bungarus fasciatus, Enhydrina schistosa, Calloselasma rhodostoma, Trimeresurus purpureomaculatus and Tropidolaemus sumatranus) was determined, based on the MTS cytotoxicity assay, which determines the survival of viable cells in monolayer MDCK and Vero cell cultures upon exposure to the snake venoms. Snake venom toxicity was expressed as the venom dose that killed 50% of the cells (CTC50) under the assay conditions. Venoms of C. rhodostoma (2.6 µg/mL, 1.4 µg/mL) and O. hannah were the most cytotoxic (3.8 µg/mL, 1.7 µg/mL) whereas N. siamensis venom showed the least cytotoxicity (51.9... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotoxicity; Snake venom; Vipers; Elapids; Kraits. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200004 |
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| Silva,Silas Arandas Monteiro e; Lacerda,Rísia; Bernegossi,Jéssica; Chorilli,Marlus; Leonardi,Gislaine Ricci. |
| ABSTRACT Liquid-Crystalline Systems represent active compounds delivery systems that may be able to overcome the physical barrier of the skin, especially represented by the stratum corneum. To obtain these systems, aqueous and oily components are used with surfactants. Of the different association structures in such systems, the liquid-crystalline offer numerous advantages to a topical product. This manuscript presents the development of liquid-crystalline systems consisting, in which the oil component is olive oil, its rheological characterizations, and the location of liquid crystals in its phase map. Cytotoxic effects were evaluated using J-774 mouse macrophages as the cellular model. A phase diagram to mix three components with different proportions... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Liquid-Crystalline Systems/development; Liquid-Crystalline Systems/reology; Drugs/skin delivery; Olive Oil/use/Liquid-Crystalline Systems; Rheology; Cytotoxicity; Bioadhesion.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100023 |
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| Rodrigues,M.; Barbosa Junior,F.; Perussi,J.R.. |
| This paper describes the effect of dipyridamole (DIP) on the cytotoxicity of cisplatin in HEp-2 human larynx cancer cells in vitro and the nature of the interaction between cisplatin and dipyridamole. Cytotoxic assays were performed to obtain the IC50 for cisplatin. The cells were treated with 0, 20, 40, 80, 120 or 200 µM cisplatin, with or without a single concentration of DIP and incubated for 60 min at 37ºC and 5% CO2 for 3 days and then counted with a hemocytometer. The accumulation of cisplatin in the cells was measured by atomic absorption and fluorescence was used to determine the membrane binding constant of DIP. In the presence of 10, 20 and 30 µM DIP, the IC50 of cisplatin was reduced by 25, 60 and 82% in HEp-2 cells. Combination index analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cisplatin; Dipyridamole; Synergism; Tumor cells; Cytotoxicity; IC50. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400017 |
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| Martínez,José Gregorio; García,Víctor Atencio; Carrasco,Sandra Pardo. |
| The endangered bocachico Prochilodus magdalenae is a native freshwater fish of Colombia, the most captured species locally and one of the most important species for ex-situ conservation (germplasm banks). The aim of this study was to examine the effect of three concentrations of Dimethylsulfoxide (DMSO) (5%, 10%, 15%) and three of glucose (305, 333, 361 mM) in the extender on spermatic DNA fragmentation (F-DNA) (by Halomax®, Chromatin dispersion) and membrane damage (D-Me) (by eosin-nigrosin staining). After assessment of sperm quality by computer analysis of motility, one part of semen from males was diluted separately with three parts of extender and filled into 0.5 ml straws. Freezing was carried out in liquid nitrogen vapor dry shipper for 30 minutes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Characiformes; Cryobiology; Cytotoxicity; Genotoxicity; Germplasm. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300011 |
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| Espinoza-Navarro,Omar; Ferreira,Carolina; Bustos-Obregón,Eduardo. |
| The earthworm Eisenia foetida (Annelida, Oligochaeta) is an excellent biosentinel model organism for assessing potential toxicological risks by xenobiotics in terrestrial ecosystems. Methamidophos is an organophosphorous insecticide widely used to control agricultural pests. Its main action is due to inhibition of the enzyme acetylcholinesterase. The aim of this study was to evaluate the toxicity of methamidophos on the morphology and male parameters in Eisenia foetida. After determining the LD50 for methamidophos (85.34 mg/kg soil), five groups were formed, including control subjects, and sprayed with tap water. Methamidophos treated groups were subjected to doses of 1/10, 1/6, 1/3 and 2/3 of the LD50. Each group was analyzed at day 1, 6, 14 and 30... |
| Tipo: Journal article |
Palavras-chave: Reproduction; Eisenia foetida; Methamidophos; Cytotoxicity. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300051 |
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| Soler,Maritza C; Molina,Jessica L; Díaz,Hugo A; Pinto,Vivian C; Barrios,Yasenka L; He,Kan; Roller,Marc; Weinstein-Oppenheimer,Caroline R. |
| Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine... |
| Tipo: Journal article |
Palavras-chave: Cimicifuga foetida; Cytotoxicity; Hsp-27; Immunofluorescence; MCF-7 cells; RT-PCR. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005 |
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| Espinoza-Navarro,O; Bustos-Obregón,E. |
| Malathion is an organophosphorous insecticide, used worldwide for pest and disease control; however, it could also affect the reproductive patterns of several species. The aim of this study was to determine the effects of malathion in the cellularity and sperm differentiation in testis and epididymis of rats. Twenty adult male Sprague Dawley rats were divided into a malathion-treated group (n=10, dose of 170 mg/kg via subcutaneous injection for a period of 13 days) and control group (n=10, injected only with normal saline). After treatments, the rats were sacrificed by regulated euthanasia and assessed for sperm count in testis and epididymis and epididymal teratospermia degree. The results showed a significant decrease in body, testicular and epididymal... |
| Tipo: Journal article |
Palavras-chave: Reproduction; Rat; Malathion; Cytotoxicity. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100020 |
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| Fabri,Rodrigo L.; Coimbra,Elaine S.; Almeida,Ana C.; Siqueira,Ezequias P.; Alves,Tânia M.A.; Zani,Carlos L.; Scio,Elita. |
| In our previous work (Fabri et al. 2009), we showed that different extracts of Mitracarpus frigidus had significant antibacterial, antifungal and leishmanicidal activities. In order to increase our knowledge about this species, this work assesses the chemical composition and the in vitro biological activity of its essential oil. Thus, the essential oil obtained by hydrodistillation of the aerial parts of M. frigidus was analyzed by GC/MS. Among several compounds detected, 11 were identified, being linalool and eugenol acetate the major components. The essential oil exhibited a moderate antibacterial effect against Staphyloccocus aureus, Bacillus cereus, Pseudomonas aeruginosa and Enterobacter cloacae (MIC 250 µg/mL). On the other hand, it showed a strong... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitracarpus frigidus; Essential oil; Antimicrobial; Antileishmanial; Cytotoxicity. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400021 |
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| Villarreal Ibarra, Edelia Claudina. |
| Ante el incremento de enfermedades crónico-degenerativas uno de los recursos terapéuticos por excelencia lo representa la medicina tradicional, la presente investigación documenta y evalúa farmacológicamente las plantas utilizadas para el control de la Diabetes Mellitus (DM) en la comunidad de Malpasito, dentro de la Reserva Ecológica de Agua Selva en Huimanguillo, Tabasco, México como un aporte a la validación científica. Registra un inventario del conocimiento tradicional de las plantas medicinales, con información sobre el uso terapéutico, forma de preparación y parte vegetal utilizada. La información se recabó mediante entrevistas estructuradas TRAMIL. Se registraron 128 especies distribuidas en 63 familias botánicas, los usos medicinales se agruparon... |
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Palavras-chave: Medicina tradicional; Diabetes; Citotoxicidad; Artemia salina; Traditional medicine; Cytotoxicity; Ciencias por Investigación; Doctorado. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2278 |
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| Registros recuperados: 116 | |
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